Abstract
Strains of Streptococcus mutans, Streptococcus sanguis and Streptococcus mitis, grown on mitis-salivarius agar, were examined for pH changes occurring in the colonies and in the medium under the colonies during aerobic and anaerobic incubation at 37 and 22 °C. Under all conditions, acid accumulation by Strep. mutans was substantially greater than that of the other organisms. Under some conditions, the pH of Strep. sanguis and Strep. mitis colonies rose during incubation, indicating that their feeble acid-producing capacity was counteracted by acid-removing or base-producing mechanisms. Terminal pH measurements of strains cultured in broth did not demonstrate interspecies differences of similar magnitude.
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