Abstract
The HCO 3 −/CO 3 2− buffer used in the bicinchoninic acid (BCA) protein assay has only weak buffering capacity at the recommended pH (11.25). Consequently the assay is rather sensitive to interference from effectively acid or alkaline samples, particularly in the micro method. Adjustment of pH in these alkaline solutions of high [Na +] is complicated by Na + errors on the pH electrode. Hence it is recommended to prepare the buffers from known amounts of NaHCO 3 and Na 2CO 3, and to reduce the pH to around 10.7; this offers much better buffering capacity with only a limited reduction in color development.
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