Abstract

In Brazil plants of Pfaffia glomerata with mosaic symptoms were found to be infected with a previously undescribed potyvirus, Pfaffia mosaic virus (PfMV). Virus particles were long and flexuous, c. 10 × 700–800 nm, and cylindrical inclusions typical of potyviruses were present in cells of infected tissue. Partial host‐range studies revealed that in addition to P. glomerata, PfMV infected only Chenopodium amaranticolor and Chenopodium quinoa. It was efficiently transmitted by the aphids Aphis gossypii and Myzus persicae. Polyclonal antiserum produced against the PfMV coat protein (CP) reacted with Potato virus Y (PVY), but not with four other potyviruses in PTA‐ELISA. The similarity of the nucleotide sequence of the PfMV coat‐protein gene (CP) varied from 7 to 76% when compared with other members of the family Potyviridae. Similarity of the 3′ NTR sequence varied from 4 to 23%. In both cases the highest similarity was with PVY. These data indicate that PfMV is a new species in the genus Potyvirus.

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