Abstract
Post-source decay (PSD) mass spectra of several peptides were obtained on a time-of-flight mass spectrometer with a curved-field reflectron to determine their amino acid sequences using very small quantities of sample. Peptides included angiotensin II, modified angiotensin II, and the tryptic fragments from somatostatin. Samples were prepared on the mass spectrometer probe using a piezo-electric micropipetting apparatus that enabled deposition of nanoliter volumes. The curved-field reflectron then enables recording of PSD mass spectra of mass-selected precursors without the need for stepping the reflectron voltage to bring different mass regions in focus. Using this instrument, sequence-specific ions could be observed with as little as a few atomoles of peptide.
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