Pesticide residues detected in Colossoma macropomum by the modified QuEChERS and GC-MS/MS methods

Abstract

This study aimed to detect pesticides in the muscle tissue of farmed tambaqui (Colossoma macropomum), in relation to good management practices (GMP) and prophylaxis and biosecurity measures. There were 54 fish farms randomly selected from the 138 found in the Microregion of Zona da Mata, RO - Brazil, for visits and collection of epidemiological data. There were extracted 24 muscle fragments were extracted from dorsolateral portion of the tail of five specimens tambaqui per fish farming. The methods of detection and quantification of pesticide residues were modified QuEChERS and GC-MS/MS. Most fish farms are small businesses and their production areas are smaller than a rural module. These ventures are an income alternative for rural producers, this information is confirmed in percentage of 88.89% (48/54) of the rural properties visited have livestock and agriculture as their main productive activity. Water monitoring was carried out in 70.37% of fish farms. However, with frequency of two water analyzes per year. Preventive and prophylactic measures taken were performed in 30% (17/54). In addition, 7% (4/54) of fish farmers reported using sodium chloride and/or using formalin as a secondary preventive measure. It is also important to mention that there was a report of administration of potassium permanganate in a fish farm, 2% (1/54). There were 12.49% (3/54) positive for at least one pesticide. The chemical compounds found Azosxystrobin (<LOQ), Epoxiconazole (<LOQ) and Chlorpyrifos (<LOQ). The presence of Azosxystrobin, Epoxiconazol and Chlorpyrifos in tambaqui muscle tissue samples was detected above acceptable limits by the PNCRC.