Pesticide-induced metabolic changes are amplified by food stress

Abstract

In natural ecosystems, long-term detrimental effects of pesticides may occur at very low concentrations, below those considered safe by the governmental risk assessment. Mechanisms potentially responsible for this unexpected sensitivity include environmental stress-factors such as food deficiency. To understand this so called “effect-paradox”, we investigated how food stress interacts with insecticide-induced biochemical fingerprints. Therefore, we measured metabolomic perturbations in Daphnia magna following a 24 h exposure to esfenvalerate under high and low food conditions. In total, 160 metabolites covering the groups of amino acids, fatty acids, lipids, and sugars were analyzed. At 0.001 μg/L esfenvalerate – a factor of >200 below the acute lethal concentration (LC50) – the endogenous metabolome was significantly affected. Further, the effect under low food conditions was considerably stronger compared to high food conditions. Individual metabolites showed up to 7-fold stronger effects under low food conditions. In general, the metabolomic changes were largely dose-specific and increased over seven days after contamination. We conclude that the metabolic profiles are altered for at least seven days after a pulse exposure, and therefore might be a key process to understanding population level changes at ultra-low pesticide concentrations in the field.