Abstract
The Chinese hickory (Carya cathayensis) is a highly prized nut crop, especially in Anhui and Zhejiang Provinces in China. In 2012 and 2013, sporadic occurrence of leaf blight was observed on C. cathayensis in Ningguo and it spread to several major Chinese hickory producing areas of Anhui Province covering more than 200 ha by 2014. Typical symptoms of Chinese hickory leaf blight included small, brown lesions that were first visible on the leaf margin, which enlarged and coalesced into the leaf becoming brown and blighted, which subsequently developed a black sporulating necrotic center surrounded by a chlorotic halo. Infected samples collected from 20 orchards in Ningguo were surface sterilized with 1.5% sodium hypochlorite for 2 min, plated on 2% potato dextrose agar (PDA) amended with streptomycin sulfate (0.1g/L), and incubated at 25°C under a 16-h/8-h light/dark cycle for 7 days. sixteen similar isolates were obtained from diseased leaves. Colonies reached 7.4 cm in average diameter after 7 days on PDA...
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