Pervasive satellite cell contribution to uninjured adult muscle fibers.

Crystal Pulliam,Bradley B. Olwin,Gabrielle Kardon,Bradley Pawlikowski,Nicole Dalla Betta

doi:10.1186/s13395-015-0067-1

Abstract

BackgroundAdult skeletal muscle adapts to functional needs, maintaining consistent numbers of myonuclei and stem cells. Although resident muscle stem cells or satellite cells are required for muscle growth and repair, in uninjured muscle, these cells appear quiescent and metabolically inactive. To investigate the satellite cell contribution to myofibers in adult uninjured skeletal muscle, we labeled satellite cells by inducing a recombination of LSL-tdTomato in Pax7CreER mice and scoring tdTomato+ myofibers as an indicator of satellite cell fusion.ResultsSatellite cell fusion into myofibers plateaus postnatally between 8 and 12 weeks of age, reaching a steady state in hindlimb muscles, but in extra ocular or diaphragm muscles, satellite cell fusion is maintained at postnatal levels irrespective of the age assayed. Upon recombination and following a 2-week chase in 6-month-old mice, tdTomato-labeled satellite cells fused into myofibers as 20, 50, and 80 % of hindlimb, extra ocular, and diaphragm myofibers, respectively, were tdTomato+. Satellite cells contribute to uninjured myofibers either following a cell division or directly without an intervening cell division.ConclusionsThe frequency of satellite cell fusion into the skeletal muscle fibers is greater than previously estimated, suggesting an important functional role for satellite cell fusion into adult myofibers and a requirement for active maintenance of satellite cell numbers in uninjured skeletal muscle.

Highlights

Adult skeletal muscle adapts to functional needs, maintaining consistent numbers of myonuclei and stem cells
Recombination in satellite cells was induced with five consecutive daily I.P. tamoxifen injections (Fig. 1a), and 6 h post-injection, the tibialis anterior (TA) muscle was harvested and recombination efficiency determined by scoring the percentage of Pax7 immunoreactive cells located underneath the basal lamina (Fig. 1b, e) that were positive for tdTomato fluorescence (Fig. 1d, e; Table 1)
Neither mononuclear tdTomato+ cells (Fig. 1k–n) nor tdTomato+ myofibers (Fig. 1k–n) were observed when Pax7Cre;tdTomato mice were injected with the vehicle demonstrating very low or nonexistent basal recombination

Summary

Introduction

Adult skeletal muscle adapts to functional needs, maintaining consistent numbers of myonuclei and stem cells. Resident muscle stem cells or satellite cells are required for muscle growth and repair, in uninjured muscle, these cells appear quiescent and metabolically inactive. To investigate the satellite cell contribution to myofibers in adult uninjured skeletal muscle, we labeled satellite cells by inducing a recombination of LSL-tdTomato in Pax7CreER mice and scoring tdTomato+ myofibers as an indicator of satellite cell fusion. Satellite cells, reported to be largely quiescent in adult muscle, were assumed to contribute minimally to adult muscle fibers unless required for injury repair or for hypertrophic growth. Satellite cell contribution to myofibers appears more extensive than previously appreciated. Up to 30 % of myofibers in uninjured hindlimb muscles acquired satellite over a 2-week interval, with satellite cell fusion varying among individual muscles [5]

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Results

Discussion

Conclusion