Abstract
The overall structure of pertussis toxoid has been established by analysis of its tryptic digest using two-dimensional liquid chromatography–tandem mass spectrometry (2D-LC–MS/MS), capillary liquid chromatography–matrix-assisted laser desorption ionization–tandem mass spectrometry (CapLC–MALDI–MS/MS), and ultraperformance liquid chromatography–mass spectrometryE (UPLC–MSE). In addition to oxidation and hydrolysis of amino acids losses of terminal peptides are observed. On-line UPLC–MSE generated a similar sequence coverage as the other two methods that involved off-line fraction collection. In light of recent favorable comparisons to data-dependent acquisition, UPLC–MSE should be the initial method of choice for analysis of a peptide mixture of moderate complexity.
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