Perturbations of gut microbiome and metabolome of pigs infected with Mycoplasma hyorhinis.

Abstract

Mycoplasma hyorhinis is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a great impact on intestinal microecology. To study the effect of M. hyorhinis infection on gut microbial composition and metabolome profile, we infected pigs with M. hyorhinis and performed metagenomic sequencing analysis of fecal sample and liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis of gut digesta. RESULTS: It is discovered that pigs infected with M. hyorhinis can enrich Sutterella and Mailhella, and deplete Dechloromonas, Succinatimonas, Campylobacter, Blastocystis, Treponema and Megasphaera. Moreover, the M. hyorhinis infected pigs had higher abundances of bacterium_0_1xD8_71, Ruminococcus_sp__CAG_353, Firmicutes_bacterium_CAG_194, Firmicutes_bacterium_CAG_534, bacterium_1xD42_87, etc., and lower abundances of Chlamydia_suis, Megasphaera_elsdenii, Treponema_porcinum, Bacteroides_sp__CAG_1060, Faecalibacterium_prausnitzii, etc. Metabolomics analysis revealed that some lipids and lipid-like molecules were increased in small intestine, while most of lipids and lipid-like molecules metabolites were decreased in large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism and thiamine metabolism. These findings demonstrate that the pigs infection with M. hyorhinis can alter the gut microbial composition and metabolites structure, which may further affect amino acid metabolism and lipid metabolism in intestine. This article is protected by copyright. All rights reserved.