PERSPECTIVE SEARCHES OF MEDICINAL PLANT RAW MATERIALS AS SOURCE OF POLYSACCHARIDES

Abstract

The purpose of the conducted researches was studying of cones of a pine ordinary as source of polysaccharides in complex processing of raw materials. Cones of a pine ordinary were assembled during pollination. For the purpose of development of complex processing of raw materials researches on studying of the cones which remained after receiving pollen are conducted. Researches showed prospects of use of cones of a pine ordinary as source of polysaccharides after receiving pollen. The pectin content in ordinary pine cones, established by the oxalate method, was 1,2 ± 0,1%. Qualitative determination of pectin is proposed by colour reaction with solution of carbazole with alcohol 0,5%. Reaction with 10% lead (II) basic acetate was also used to identify pectin. One indicator of the detoxifying activity of pectin is the degree of esterification (ratio of esterified and free carboxyl groups). Low esterified pectins (esterification rate below 50%) are used as detoxicant. Important is the fact that the complex processing of ordinary pine cones will make it possible to obtain pectin actually from pollen production waste. In the developed scheme of complex processing of ordinary pine cones for hydrolysis-extraction of pectin-high substances from raw materials, it is proposed to use citric acid solution with pH 1,8-2,0. Methods for identifying and quantifying pectin in an object are valid. As a result of all the studies, a method was developed for producing pectin from ordinary pine cones in the complex processing of raw materials, the content of which was 1,2±0,1%, and the degree of esterification 13,20 ± 0,5%. The results of the conducted studies showed that the content of tannins in the studied raw materials of pine cones was 0,66±0,02%. As a result of all studies, a method of producing pectin from ordinary pine cones in complex processing of raw materials has been developed. The obtained results make it possible to recommend the obtained pectins as an effective detoxifying agent and a source of polysaccharides, tannins.