Abstract

Barcode technology has a broad spectrum of applications including healthcare, food security, and environmental monitoring, due to its ability to encode large amounts of information. With the rapid development of modern molecular research, barcodes are utilized as a reporter with different molecular combinations to label many biomolecular targets, including genomic and metabolic elements, even with multiplex targeting. Along with the advancements in barcoded bioassay, the improvements of various designs of barcode components, encoding and decoding strategies, and their portable adoption are indispensable in satisfying multiple purposes, such as medical confirmation and point-of-care (POC) testing. This perspective briefly discusses the current direction and progress of barcodes development and provides a hypothesis for barcoded bioassay in the near future.

Highlights

  • A barcode represents a collection of data which could be decoded by an optical readout [1]

  • We provide a summary about the different types of barcodes, and predictions

  • The common barcodes used for bioassay could be categorized into two main classes: Fluorescent

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Summary

Introduction

A barcode represents a collection of data which could be decoded by an optical readout [1]. New types of barcodes, known as 2D recognition code, with or without fluorescence, used to label target molecules such as proteins, DNA, and RNA. These barcodes are are widely used to label target molecules such as proteins, DNA, and RNA. These applied for detecting and monitoring the biological activity in vitro and in vivo for bioanalysis [14,15]. Barcodes are applied for detecting and monitoring the biological activity in vitro and in vivo for In the future, these advantages of different barcodes could be upgraded to achieve many different bioanalysis [14,15].

Classification of Barcodes for Bioassay
Aby string-like fluorescent barcodeof dye-labeled
A string-like fluorescent barcode dye-labeled
Perspective on Barcodes
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