Perspective for using 2,6-dimethylpyridine-N-oxide to reduce the toxic effect of xenobiotics in mammals ‎

Olesia Vasetska,Victoria Tsygankova,Petro Zhminko,Mykola Prodanchuk,Anatilyi Galkin

doi:10.51847/txcxi0pso1

Abstract

This work aimed to study the isolated and subsequent effects of 2,6-Dimethylpyridine-N-oxide (Ivin) and 2,4-D-2-EHE on the survival of rats and the population characteristics of the total mRNA that implements molecular genetic processes in rats. The studies on Wistar Han (SPF) rats were performed. It was found that Ivin at doses of 13 and 0.013 mg/kg bw, corresponding to 1/100 and 1/100000 DL50, does not cause clinical signs of intoxication in rats. Ivin increases the level of hybridization in m

Highlights

This work aimed to study the isolated and subsequent effects of 2,6-Dimethylpyridine-N-oxide (Ivin) and 2,4-D-2-EHE on the survival of rats and the population characteristics of the total mRNA that implements molecular genetic processes in rats
Taking into account the population differences of mRNA molecules in this work we investigated levels of homology between populations of cytoplasmic mRNA isolated from the liver and blood of experimental and control rats that were determined according to the degree of hybridization (%) between cytoplasmic mRNA isolated from control rats with [ɑ32P]-cDNA from experimental rats using dot-blot hybridization method [3, 24,25,26]
When rats were administered with 2,4-D-2-EHE at doses of 540 mg/kg bw in combination with Ivin at doses of 13 mg/kg bw and 0.013 mg/kg bw (Figures 5 and 6), the degree of hybridization between cytoplasmic mRNA isolated from control and [ɑ-32P]cDNA from experimental rat liver and blood cells was reduced but to a lesser degree than after isolated administration of 2,4-D-2-EHE, compared to the control

Summary

Introduction

This work aimed to study the isolated and subsequent effects of 2,6-Dimethylpyridine-N-oxide (Ivin) and 2,4-D-2-EHE on the survival of rats and the population characteristics of the total mRNA that implements molecular genetic processes in rats. After the action of 2,4-D-2-EHE to rats at a dose of 540 mg/kg bw (corresponding to 1 DL50), pronounced intoxication is observed after 6 hours with the following 40% lethal of the animals. Based on the results obtained, the possible molecular genetic mechanisms of action of the studied compounds are discussed. In the scientific literature on many chemicals, the mechanisms of their toxic effects on plants and animals are describes in detail. For example, intensive technologies based on the application of physiologically active compounds of natural and synthetic origin to increase crop yields and their resistance to abiotic and biotic stress are widely used. Researches of the mechanisms of action of plant biostimulants based on 2,6-dimethylpyridine-N-oxide showed

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