Personalising wound care: A gene expression signature for predicting clinical outcomes of venous leg ulcers

Abstract

s / International Journal of Surgery 23 (2015) S15eS134 S20 characteristics on a suitable polymer with ideal bulk properties provides a basis for further research into the effects of PSM on cell behaviour. 0171: PERSONALISING WOUND CARE: A GENE EXPRESSION SIGNATURE FOR PREDICTING CLINICAL OUTCOMES OF VENOUS LEG ULCERS D. Bosanquet, A. Sanders, E. Cox, F. Ruge, J. Lane, K. Harding, W. Jiang. University Hospital of Wales, UK Aim: Despite their widespread occurrence, sensitive prognostic markers of chronic venous leg ulcers VLUs are noticeable by their absence. We describe a novel gene expression signature of wound edge tissue in VLUs which allows accurate personalised outcome modelling, permitting individually tailored treatments. Methods: Sequential refinement and testing of a gene signature was developed utilising three distinct cohorts of humanwound tissue. Over 111 pre-selected candidate genes were first screened using a cohort of acute and chronic wound tissue (n 1⁄4 24) by way of quantitative PCR. Genes showing significant differences were combined and examined as part of a controlled prospective study of 71 patients with VLUs. The final signature was evaluated using a prospective, blinded study comprising 85 consecutive patients with VLUs. Results: The initial gene signature comprised 24 genes (WD24) that allowed distinction between healing wound from non-healing wounds (p < 0.0001, sensitivity: 40%, specificity: 98%). Subsequent refinement excluded 10 genes to create a final 14 gene signature (WD14) which demonstrated significant prognostic power in a prospective, blinded study (p < 0.00001, sensitivity: 84%, specificity: 74%). Conclusion: We report a novel gene signature that can predict wounds at low propensity to heal with current treatment strategies. Advanced wound care products and therapies may be of particular benefit to this population. 0227: AN INCREASED POST-OPERATIVE INFLAMMATORY RESPONSE IS ASSOCIATED WITH REDUCED SURVIVAL FOLLOWING RESECTION OF COLORECTAL LIVER METASTASES O. Sogaolu, H. Shaker, P. Snape, D. O'Reilly. Central Manchester Foundation