Abstract
AbstractThe methanolic extract of Tylophora asthamatica (Asclepiadaceae) leaves was bioassayed by a leaf disc method for feeding deterrence using Spilosoma obliqua Walker as the test insect. Tylophorine, isolated from the extract was evaluated under laboratory and greenhouse conditions for its feeding deterrence, persistence and systemic properties by using the natural host plant (Ricinus communis) of the test insect. The antifeedant compound azadirachtin and crude neem extract were used as standards. Tylophorine produced complete inhibition of feeding under laboratory conditions and was persistent in field trials for two days as determined by the damage rating score. However, both tylophorine and the crude neem extract were shown to be nonsystemic when tested in the Hoagland culture media.
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