Persistence of Xanthomonas campestris pv. campestris in Field Soil in Central Europe.

Filip Gazdik,Steven J. Roberts,Dariusz Grzebelus,Miroslav Baranek,Jana Cechova,Robert Pokluda,Samuel Magnus,Rafal Baranski,Ales Eichmeier

doi:10.3390/microorganisms9030591

Filip Gazdik, Steven J. Roberts + Show 7 more

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https://doi.org/10.3390/microorganisms9030591

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Abstract

Xanthomonas campestris pv. campestris (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses of brassica crop production usually result from seed-borne infection, but carry-over of inoculum in field soil may also be possible. The aim of this study was to monitor persistence of Xcc in field soil in central Europe using a conventional PCR assay with hrpF primers and a two-step nested real-time PCR assay using Zur primers. The work has demonstrated that nested real-time PCR can be used to improve the analytical sensitivity for detection of Xcc in soil compared to conventional PCR, and that Xcc may persist in soil for up to two years following an infected brassica crop in central European climatic conditions.

Highlights

Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), causes black rot of crucifers [1] and has a worldwide distribution [2]
Various studies have been made regarding the persistence of Xcc in soil, but most of them have focused on survival in association with crop debris
The DNA concentration in the soil extracts ranged from 4 to 243 ng·mL−1, but there was no consistent relationship with the positivity by either conventional or nested real-time

Summary

Introduction

Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), causes black rot of crucifers [1] and has a worldwide distribution [2]. Campestris (Pammel) Dowson (Xcc), causes black rot of crucifers [1] and has a worldwide distribution [2]. It is considered one of the most important diseases of brassicas [1,3] and may result in significant losses in brassica crop production [2]. Xcc has been reported to survive in plant debris in soil for up to two years in the Netherlands [11], but there is no data for central Europe. Schaad & White [9] suggests that Xcc cannot survive in soil for more than 42 days in winter and 14 days in summer without the host plant debris

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