Persistence and expression of the plasmid pBR322 inEscherichia coli K12 cultured in complex medium

Abstract

The stability and gene expression of plasmid pBR322 in a chemostat with complex non-selective medium at different dilution rates were studied. It was observed that pBR322 was eventually lost from the population after a long lag period. The rate of plasmid loss decreases with decreasing dilution rate. This result is different from those obtained with cells grown in defined medium, where plasmid loss was observed to decrease with increasing dilution rate. In addition, it was observed that the β-lactamase activity per ml per optical density of cell culture, independent of the dilution rates, increases with time and reaches a maximum around 4.5 units after 100 hrs of continuous culture.