Abstract
To investigate the capacities of persistence and dissemination of blaNDM-5 within Escherichia coli and in aquatic environment, we characterized E. coli (sequence type 636) strains B26 and B28 isolated one month apart from the same urban river in Montpellier, France. The two isolates carried a pTsB26 plasmid, which sized 45,495 Kb, harbored blaNDM-5 gene and belonged to IncX-3 incompatibility group. pTsB26 was conjugative in vitro at high frequency, it was highly stable after 400 generations and it exerted no fitness cost on its host. blaNDM-5harboring plasmids are widely dispersed in E. coli all around the world, with no lineage specialization. The genomic comparison between B26 and B28 stated that the two isolates probably originated from the same clone, suggesting the persistence of pTsB26 in an E. coli host in aquatic environment.
Highlights
Antimicrobial resistance (AMR) occurs worldwide and the World Health Organization has identified it as one of the three main threats to human health [1,2]
We described pTsB26, an IncX-3 plasmid encoding blaNDM-5 gene carried by two strains of E. coli isolated from an urban river one month apart
The B26 genome is 4927 Mb and includes four plasmids belonging to IncFIA/FIB, IncFII, IncQ1 and IncX-3 incompatibility groups
Summary
Antimicrobial resistance (AMR) occurs worldwide and the World Health Organization has identified it as one of the three main threats to human health [1,2]. AMR, with consequences for antimicrobial treatments failures [3]. The environment is considered as a main player in the emergence and diffusion of AMR. Urban and rural surface waters constitute hotspots for exchanges among microorganisms of human and environmental origin, which are all subject to strong selection pressures due to diverse pollutions [3,5]. Β-lactams are by far the most widely consumed antibiotics worldwide [9], and among β-lactams, carbapenems are last resort treatment for multidrug-resistant bacterial infections [10,11,12]. Several studies reported the occurrence of Carbapenemase Encoding Genes (CEGs) in aquatic environments [6,7,8]
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