Abstract
The feasibility of employing peroxyoxalate chemiluminescence (PO-CL) detection in capillary electrophoresis (CE) was demonstrated using a two- step approach for the CE separation and dynamic elution (elution under pressure) of the analytes. In this approach, potential problems associated with incompatibilities between mixed aqueous-organic solvent and clectrically driven separation systems were avoided by switching off the CE power supply at an appropriate time and connecting the CE capilliary to a syringe pump to effect dynamic elution. The effect of dynamic flow- rate and PO-CL reagent concentration on the CL signal intensity and/or peak width were examined for the measurements of three dansylated amino acids. The average limit of detection for these analytes using this PO-CL method is about 1.2 fmol ( ca. 85 nM) which is approximately 35-fold lower than UV absorption methods.
Published Version
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