Abstract
Background: Peroxisome proliferator-activated receptors (PPARs) are transcription factors that belong to the superfamily of nuclear hormone receptors. Three PPARs members, PPAR-alpha, PPAR- delta and PPAR gamma, have been characterized. PPAR-delta in humans is encoded by the PPARD gene and has been shown to possess a role in regulating fatty acid catabolism and energy homeostasis. Several polymorphisms have been identified in PPARD as potential functional variants. PPARD +294T > C polymorphism was shown to modify serum lipid concentrations in some ethnic groups. Aim: The current study aimed to screen the presence of the PPARD polymorphism in the Saudi population. Method: Blood samples were collected from normal individuals. Genomic DNA was extracted from peripheral blood monnuclear cells and the partial gene of the PPARD was amplified by polymerase chain reaction and subjected to gel electrophoresis. PPARD rs2016520 polymorphism was analyzed by polymerase chain reaction–restriction fragment length polymorphism. Results: The frequencies of CC, CT, and TT genotypes in normal Saudi population were 9.09%, 14.2% and 76.6%, respectively. Conclusion: The Saudi nationals seems to have low frequency of the PPARD +294T > C polymorphisms. Limitations: Relatively small sample size, serum lipid levels were not determined.
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