Peroxiredoxin 1 controls β-catenin activity and Epstein-Barr Virus-encoded MicroRNA in Nasopharyngeal Carcinoma

Abstract

Abstract BACKGROUND Nasopharyngeal Carcinoma(NPC) is a rare tumor in the world, but high in some countries such as southern China, Mediterranean Africa and Middle East. According to studies, Epstein-Barr virus is affected more aggressive in NPC. In NPC, EBV-miR-BARTs have been demonstrated, especially EBV-miR-BART17 have relation to β-catenin activity but the studies are still unclear. Peroxiredoxin 1 (Prdx1) is an antioxidant enzyme and is highly susceptible to oxidative stress. METHODS To study the roles of Prdx1 and EBV-miR-BART17-5p in NPC C666-1 and HK1 cells, it is transfected to siRNA of Prdx1 and then it is measured using RT-PCR for RNA level and western blot for protein level. Colony forming assay and Wound-healing assay were used to detect the cell proliferation in NPC cells. RESULT EBV-miR-BART17-5p expression was higher in C666-1 than HK1 cells. The expression of Prdx1 is different between C666-1 and HK1 cells because of EBV genes. EBV-miR-BART17 expression was found to relate to cell proliferation through targeting NF-κB signaling pathway. CONCLUSION These data suggested that Prdx1 and EBV-miR-BART17-5p play roles in inducing β-catenin activity in NPC cells. Our findings suggest that the regulating of Prdx1 is an important therapeutic target for NPC.