Abstract
Peroxisomes are essential organelles that play important roles in a variety of biological processes in eukaryotic cells. To understand the synthesis of peroxisomes comprehensively, we identified the gene FgPEX22-like, encoding FgPEX22-like, a peroxin, in Fusarium graminearum. Our results showed that although FgPEX22-like was notably different from other peroxins (PEX) in Saccharomyces cerevisiae, it contained a predicted PEX4-binding site and interacted with FgPEX4 as a rivet protein of FgPEX4. To functionally characterize the roles of FgPEX22-like in F. graminearum, we performed homologous recombination to construct a deletion mutant (ΔPEX22-like). Analysis of the mutant showed that FgPEX22-like was essential for sexual and asexual reproduction, fatty acid utilization, pathogenicity, and production of the mycotoxin deoxynivalenol. Deletion of FgPEX22-like also led to increased production of lipid droplets and decreased elimination of reactive oxygen species. In addition, FgPEX22-like was required for the biogenesis of Woronin bodies. Taken together, our data demonstrate that FgPEX22-like is a peroxin in F. graminearum that interacts with PEX4 by anchoring PEX4 at the peroxisomal membrane and contributes to the peroxisome function in F. graminearum.
Highlights
IntroductionFusarium head blight (FHB), caused by F. graminearum (teleomorph Gibberella zeae), is an acute disease of wheat and barley worldwide
Fusarium head blight (FHB), caused by F. graminearum, is an acute disease of wheat and barley worldwide
We identified a new peroxin in F. graminearum, FgPEX22-like, which has a PEX4-binding site and can directly interact with FgPEX4
Summary
Fusarium head blight (FHB), caused by F. graminearum (teleomorph Gibberella zeae), is an acute disease of wheat and barley worldwide. In eukaryotic cells, peroxisomes are involved in methanol oxidation, disposal of ROS, and utilization of carbon sources (Lazarow and Fujiki, 1985; Wanders, 2004; Gould et al, 2010). Peroxisomes are the unique sites of fatty acid β-oxidation (Hiltunen et al, 2003), whereas in plants, they are essential for host resistance, embryo development, synthesis of phytohormones, and the glyoxylate cycle (Hu et al, 2012; Shabab, 2013). Peroxisomes are involved in several physiological processes in mammals, such as the synthesis of cholesterol, plasmalogens, and bile acids (Wanders and Waterham, 2010).
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