Abstract
The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA using Ganoderma lucidum polysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.
Highlights
As we all know, dopamine (DA) is an important catecholamine neurotransmitter in the nervous system, which plays a key role in human metabolism
In summary, Ganoderma lucidum polysaccharide (GLP) was used as a reducing agent and stabilizer to synthesize platinum nanoclusters
The catalytic kinetic process of Pt600-GLP NCs conformed to the typical Michaelis-Menten equation, and their affinity for both substrates was bigger than that of horseradish peroxidase (HRP)
Summary
Dopamine (DA) is an important catecholamine neurotransmitter in the nervous system, which plays a key role in human metabolism. Some substances with peroxidase-like properties have been considered to be essential in colorimetric sensor platforms It overcomes the shortcomings of natural horseradish peroxidase (HRP), such as high cost, poor stability, difficult preparation, and storage. A variety of substances with peroxidase-like properties sprung up like mushrooms, from noble metal nanoparticles [6], metal oxides [7], to multi-metal compounds [8] This peroxidase-like activity of nanozymes has been used in colorimetry to detect many biologically related substances, such as H2O2 [9], glucose [10], DA [11], and glutathione [12]. This method had a good performance in detecting DA in real samples
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