Peroxidase-like activity of bimetal Cu-Zn oxide mesoporous nanospheres for the determination of o-aminophenol.

Abstract

To enhance the peroxidase-like performance and its application in detection of toxic o-aminophenol (o-AP), a kind of bimetal Cu-Zn oxide-based mesoporous nanosphere (Cu2/3Zn1/3O PNPs) was constructed under microwave-radiation conditions. Its mesoporous microstructure and peroxidase-like catalytic activity were investigated in detail. The results showed that Cu2/3Zn1/3O PNPs possessed a high specific surface area of 34.89 m2g-1 and a well-distributed mesoporous size of approximate 6.07nm, which endowed the superior peroxidase-like performance. The materialcatalyzes the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) with Km/Vmax of 0.104mM/3.79 × 10-8M·s-1 in the presence of H2O2. Especially o-AP could exclusively deteriorate the characteristic UV-Vis absorbance intensity at 653nm (A653) of the Cu2/3Zn1/3O PNPs-TMB-H2O2 system with obvious color change from blue to colorless. Under the optimal conditions, the effect of some interfering substances was low and the limit of detection (LOD) for o-AP was 1.65 × 10-8mol/L (S/N = 3). When applied to thecolorimetric detection of o-AP in practice, the recovery was between 96.1 and 107.2% with R.S.D. less than 2.04%. The mechanism of synergic-enhancement peroxidase-mimic activity of Cu2/3Zn1/3O PNPs and its exclusive colorimetric response to o-AP were proposed as well.