Peroxidase-triggered formation of fluorescent peptide-based nanoarchitectonics

Abstract

Fluorescent dye labeled peptides and oligonucleotides are important tools for biochemical and cellular research. Dityrosine bond is easily formed in vitro and shows blue fluorescence, so it can be used as a useful symbol of oxidation conditions in biological systems. The formation of dityrosine can be carried out by enzymatic reaction, Fenton reaction and photoinitiation reaction. The enzymatic reaction has attracted much attention because of its mild reaction conditions, which can be performed at room temperature and pressure, and the reaction rates are faster than the corresponding non-catalytic reaction. In this study, peroxidase is selected to catalyze the dipeptides containing tyrosine, resulting nanostructures containing dipeptides with stable blue fluorescence under mild conditions. Nanostructured peptides with stable fluorescence can be applied to fluorescent labeling, and the reaction catalyzed by enzymes to produce blue fluorescence can be used as candidates for lighting inflammatory cells and designing visual materials.