Abstract
Abstract— The influence of pH and concentration of reagents on the chemiluminescence emitted during peroxidase mediated oxidation of phenol derivatives was studied. Maximal light emission was determined under conditions where chemiluminescence due to auto‐oxidation was negligible. With phloroglucinol and purpurogallin as substrates, a direct proportionality was obtained between the concentration of peroxidase and the maximal light emission. p‐Phenylenediamine enhances 8‐fold the light emitted with purpurogallin. With resorcinol as substrate the relation between concentration of enzyme and maximal light emission gives an S‐shaped curve. With pyrogallol the light emitted is proportional to the square of the concentration of peroxidase.
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