Peroxidase and isoperoxidase changes in relation to root initiation of Asparagus cultured in vitro

Abstract

Rhizogenesis of Asparagus stem segments in vitro is accompanied by a decrease in total peroxidase activity. The activity of the two extreme anodic isoperoxidases increases while that of the four most cathodic ones decreases. These biochemical events take place in the upper part of the cutting as well as in the rooting region. Cuttings from Asparagus plants which root more easily than others can be distinguished by their higher peroxidase activity in parallel with a greater activity in their anodic (the two extreme ones) and cathodic (the four extreme ones) isoperoxidases. It is suggested that this type of peroxidase analysis may be used as a test for the selection of mother plants with a high root-forming ability.