Abstract
Egg yolk phosphatidylcholine monolamellar liposomes (1000 Å in diameter) loaded with cytochrome c were placed into an external solution, in which superoxide radicals, O 2 −, were generated by a xanthine-xanthine oxidase system. The penetration of the superoxide radicals across the liposomal membrane was detected by cytochrome c reduction in the inner liposome compartment. The effects of modifiers and temperature on this process were studied. The permeability of liposomal membrane for O 2 −( P′ O 2 − (7.6 ± 0.3) · 10 -8 cm/s), or HO 2 • ( P′ HO 2 − 4.9 · 10 -4 cm/s) were determined. The effect of the transmembrane electric potential (K + concentration gradient, valinomycin) on the permeability of liposomal membranes for O 2 − were investigated. It was found that O 2 − can penetrate across liposomal membrane in an uncharged form. The feasibility of penetration of superoxide radicals through liposomal membrane, predominantly via anionic channels, was demonstrated by the use of an intramolecular cholesterol-amphotericin B complex.
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