Abstract
Comamonas testosteroni KF-1 is a model organism for the elucidation of the novel biochemical degradation pathways for xenobiotic 4-sulfophenylcarboxylates (SPC) formed during biodegradation of synthetic 4-sulfophenylalkane surfactants (linear alkylbenzenesulfonates, LAS) by bacterial communities. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 6,026,527 bp long chromosome (one sequencing gap) exhibits an average G+C content of 61.79% and is predicted to encode 5,492 protein-coding genes and 114 RNA genes.
Highlights
Comamonas testosteroni strain KF-1 (DSM14576) was isolated for its ability to degrade xenobiotic sulfophenylcarboxylates (SPC), which are degradation intermediates of the synthetic laundry surfactants linear alkylbenzenesulfonates (LAS) [1]
We present a summary classification and a set of features for another C. testosteroni strain, strain KF-1, which has been genome-sequenced in order to improve the understanding of the molecular basis for its ability to degrade xenobiotic compounds, xenobiotic, chiral 3-C4-SPC, and how this novel degradation pathway has been assembled in this organism, together with the description of its draft genome sequence and annotation
C. testosteroni KF-1 is a rod-shaped Gram-negative bacterium that can be motile and grows strictly aerobically with complex medium or in a prototrophic manner when cultivated in mineral-salts medium [58] with a single carbon source
Summary
Comamonas testosteroni strain KF-1 (DSM14576) was isolated for its ability to degrade xenobiotic sulfophenylcarboxylates (SPC), which are degradation intermediates of the synthetic laundry surfactants linear alkylbenzenesulfonates (LAS) [1]. Commercial LAS is completely biodegradable, as known for more than 50 years [3], e.g., in sewage treatment plants, and its degradation is catalyzed by heterotrophic aerobic bacterial communities in two steps. The ultimate degradation step, i.e., mineralization of all SPCs, is catalyzed by other bacteria in the community, and one representative of these is Comamonas testosteroni KF-1. Strain KF-1 was isolated from a laboratory trickling filter that had been used to enrich a bacterial community from sewage sludge that completely degraded commercial LAS and SPCs [1,6]. The genome of C. testosteroni ATCC 11996T has been sequenced in order to further improve the understanding of the molecular basis for the degradation of steroids [22]
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