Abstract
We investigated the effects of age, sex and strain in the induction of peritoneal exudate colony-forming cells (PE-CFC) in mice. Sex and age (ranging from 3 weeks to 12 months) had no significant effect on the induction of PE-CFC. However, we found significant difference between strains in terms of the number of nucleated cells and the proportion of PE-CFC in exudate cells. When we investigated the mechanisms behind this strain difference, we found that it was due to neither the type of colony-stimulating factor employed in culture, nor the type of stimulants used to induce the exudate, nor the difference in the kinetics of appearance of PE-CFC in the peritoneal cavity. We also studied the induction of PE-CFC in rats and hamsters and the growth of these cells in vitro. Unlike mouse cells, PE-CFC from rats and hamsters could also use media conditioned by cells from other species as a source of colony-stimulating factor.
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