Periplaneta americana extract promotes intestinal mucosa repair of ulcerative colitis in rat.

Nan-nan Xue,Jun-zhu Wu,Xiu-mei Wu,Miao He,Wen-wen Du,Cheng-gui Zhang,Qi-yan Li,Zi-zhong Yang,Huai Xiao,Yue Li

doi:10.1590/s0102-865020200100000002

Nan-nan Xue, Jun-zhu Wu + Show 8 more

Open Access

https://doi.org/10.1590/s0102-865020200100000002

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Abstract

Purpose:To investigate the mechanism of Periplaneta americana extract promoting intestinal mucosal repair of OXZ-induced colitis in rat.Methods:All experiments used an equal number of male and female SD rats (n=48). We injected OXZ into the colon to induce UC rat model. To determine the optimal concentration of P. Americana's extract (PA-40), it was classified into low (L), medium (M), and high (H) doses. After OXZ treatment, each drug was administered by enema for 7 consecutive days. Rats were divided into the following 6 groups: (1) Saline treatment group (NC), (2) OXZ treatment UC model group (MC), (3) OXZ + budesonide group (BUN), (4) OXZ + PA-40 L group, (5) OXZ + PA-40 M group, (6) OXZ + PA-40 H group. Disease activity index (DAI) scores, colon length, histopathological score, serum cytokine level (IL-4, IL-10, iNOS, tNOS), and amount of MPO, EGF, IL-13 in colonic mucosa were measured.Results:PA treatment had a significant healing effect on the OXZ-colitis model and significantly reduced the lesioned area, especially in the PA-40H groups. PA treatment did not alter the expression of IL-10 and MPO level, but increased EGF (epidermal growth factor) and decrease IL-13 in the colonic tissue. PA inhibited the rise of NOSs (nitric oxide synthase) and decreased the serum IL-4 level.Conclusions:The data suggest that Periplaneta americana extract may be a potential compound for the treatment of colonic lesions. The mechanism may be related to inhibiting the secretion of IL-13 and promoting the formation of EGF.

Highlights

Inflammatory bowel disease (IBD) is a group of inflammatory diseases that affect the gastrointestinal tract, including Crohn’s disease (CD) and ulcerative colitis (UC)[1]
The occult blood kit, MPO, Nitric oxide synthase (NOS) test kits and rat interleukin-13 ELISA kit were purchased from jiangsu nanjing jiancheng biotechnology CO
Rat interleukin-4 (IL-4), interleukin-10 (IL-10) and epidermal growth factor (EGF) ELISA kits were purchased from xinbosheng biotechnology CO

Summary

Introduction

Inflammatory bowel disease (IBD) is a group of inflammatory diseases that affect the gastrointestinal tract, including Crohn’s disease (CD) and ulcerative colitis (UC)[1]. UC is characterized by continuous inflammation of the lamina propria of the colon, accompanied by mucosal barrier damage and infiltration of inflammatory factors[2]. Clinical symptoms are abdominal pain, diarrhea, weight loss, mucopurulent blood stools and some common complications such as colon cancer[3]. It is the most common form of inflammatory bowel disease worldwide[4]. There are no treatments for reducing inflammation and restoring intestinal barrier function[10]. Studies at home and abroad have shown that its induced colitis is similar to human UC in histological features and inflammatory distribution[15,16]

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