Abstract
Nerve crush injury is a well-established axonotmetic model in experimental regeneration studies to investigate the impact of various pharmacological treatments. Hericium erinaceus is a temperate mushroom but is now being cultivated in tropical Malaysia. In this study, we investigated the activity of aqueous extract of H. erinaceus fresh fruiting bodies in promoting functional recovery following an axonotmetic peroneal nerve injury in adult female Sprague-Dawley rats by daily oral administration. The aim was to investigate the possible use of this mushroom in the treatment of injured nerve. Functional recovery was assessed in behavioral experiment by walking track analysis. Peroneal functional index (PFI) was determined before surgery and after surgery as rats showed signs of recovery. Histological examinations were performed on peroneal nerve by immunofluorescence staining and neuromuscular junction by combined silver-cholinesterase stain. Analysis of PFI indicated that return of hind limb function occurred earlier in rats of aqueous extract or mecobalamin (positive control) group compared to negative control group. Regeneration of axons and reinnervation of motor endplates in extensor digitorum longus muscle in rats of aqueous extract or mecobalamin group developed better than in negative control group. These data suggest that daily oral administration of aqueous extract of H. erinaceus fresh fruiting bodies could promote the regeneration of injured rat peroneal nerve in the early stage of recovery.
Highlights
Peripheral nerve problems are common and encompass a large spectrum of traumatic injuries, diseases, tumors and iatrogenic lesions
We investigated the activity of aqueous extract of H. erinaceus fresh fruiting bodies in promoting functional recovery following an axonotmetic peroneal nerve injury in adult female Sprague-Dawley rats by daily oral administration
Analysis of Peroneal functional index (PFI) indicated that return of hind limb function occurred earlier in rats of aqueous extract or mecobalamin group compared to negative control group
Summary
Peripheral nerve problems are common and encompass a large spectrum of traumatic injuries, diseases, tumors and iatrogenic lesions. Functional nerve defects have been remedied by many methods, including nerve transfer, nerve grafts, artificial nerve conduit bridging and end-to-side neurorrhaphy [3]. These methods only provide a regenerative environment for injured nerves. Recovery of function depends on various local and systemic factors. Regeneration of axons from the proximal stump of an injured nerve to the distal nerve stump is one of the most important factors in reinnervation of peripheral tissue. Recent studies have shown that locally applied neurotrophins can enhance survival of damaged neurons and regrowth of lesioned axons in the central and peripheral nervous systems in rats [4]. The beneficial effect of systemically administered neurotrophins on axonal regeneration is largely
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