Peripheral chemoreflex contribution to ventilatory long-term facilitation induced by acute intermittent hypercapnic hypoxia in males and females.

Abstract

Ventilatory long-term facilitation (vLTF) refers to respiratory neuroplasticity that develops following intermittent hypoxia in both healthy and clinical populations. A sustained hypercapnic background is argued to be required for full vLTF expression in humans. We determined whether acute intermittent hypercapnic hypoxia elicits vLTF during isocapnic-normoxic recovery in healthy males and females. We further assessed whether tonic peripheral chemoreflex drive is necessary and contributes to the expression of vLTF. Following 40min of intermittent hypercapnic hypoxia, minute ventilation was increased throughout 50min of isocapnic-normoxic recovery. Inhibition of peripheral chemoreflex drive with hyperoxia attenuated the magnitude of vLTF. Males and females achieve vLTF through different respiratory recruitment patterns. Ventilatory long-term facilitation (vLTF) refers to respiratory neuroplasticity that manifests as increased minute ventilation ( ) following intermittent hypoxia. In humans, hypercapnia sustained throughout intermittent hypoxia and recovery is considered necessary for vLTF expression. We examined whether acute intermittent hypercapnic hypoxia (IHH) induces vLTF, and if peripheral chemoreflex drive contributes to vLTF throughout isocapnic-normoxic recovery. In 19 individuals (9 females, age: 22±3years; mean±SD), measurements of tidal volume (VT ), breathing frequency (fB ), , and end-tidal gases ( and ), were made at baseline, during IHH and 50min of recovery. Totalling 40min, IHH included 1min intervals of 40s hypercapnic hypoxia (target =50mmHg and =+4mmHg above baseline) and 20s normoxia. During baseline and recovery, dynamic end-tidal forcing maintained resting and and delivered 1min of hyperoxia ( =355±7mmHg) every 5min. The depression in during hyperoxia was considered an index of peripheral chemoreflex drive. Throughout recovery was increased 4.6±3.7lmin-1 from baseline (P<0.01). Hyperoxia depressed at baseline, and augmented depression was evident following IHH (Δ =-0.8±0.9 vs. -1.7±1.3lmin-1 , respectively, P<0.01). The vLTF was similar between sexes (P=0.15), but males had larger increases in VT than females (sex-by-time interaction, P=0.03), and females tended to increase fB (P=0.09). During isocapnic-normoxic recovery following IHH: (1) vLTF is expressed in healthy humans; (2) vLTF expression is attenuated but not abolished with peripheral chemoreflex inhibition by hyperoxia, suggesting a contribution from central nervous pathways in vLTF expression; and (3) males and females develop similar vLTF through different ventilatory recruitment strategies.