Peripheral Blood Stem Cell Autografts

Abstract

I. Introduction.- Peripheral Blood Stem Cells: 1909 to the Nineties.- II. Biological Background.- Transplants of Blood-Derived Hematopoietic Cells.- Biology of Hematopoietic Stem Cells.- Stroma and Hematopoiesis.- Mobilization of Stem Cells and Progenitors After Thiamphenicol-lnduced Hematopoietic Stress.- Stimulation of Granulocyte Macrophage Progenitors via Monocyte/Macrophage Activation: A Fundamental Regulatory Pathway of Terminal Differentiation.- The Use of Long-Term Culture Techniques to Study Peripheral Blood Stem Cells Before and After Autologous Transplantation.- Peripheral Blood Stem Cell Transplantation: Predictions from Studies in the Mouse.- Production of Haematopoietic and Immune System Growth Factors After Marrow Transplantation and Their Use in Enhancing Haematopoietic and Immune Reconstitution.- Ex Vivo and in Vivo Cytokine Studies in Irradiated Animals.- Theoretical and Practical Considerations on the Use of Recombinant Human GM-CSF in Transplantation.- III. Methods.- Identification of CD34-Positive Cells by Multiparameter Flow-Cytometry.- Short Term Colony Assay for CFU-GM.- Stem Cell Mobilization by Myelosuppressive Chemotherapy.- Clinical Usefulness and Optimal Harvesting of Peripheral Blood Stem Cells Mobilized by High Dose Cyclophosphamide and Recombinant Human GM-CSF.- Mobilization of Blood Stem Cells with Recombinant Human (rh) G-CSF in Patients with Hematological Malignancies and Solid Tumors.- IL-3 plus GM-SCF Induced Recruitment of High Numbers of Peripheral Blood Progenitor Cells Following Standard Dose Chemotherapy.- Peripheral Blood Stem Cell Mobilization in the Outpatient Setting.- Collection of Peripheral Blood Stem Cells Apheresis Monitoring and Procedure.- Collection of Peripheral Blood Stem Cells for Autografts in Children with Cancer.- Cryopreservation and Infusion of Peripheral Blood Stem Cells.- IV. Clinical Aspects of PBSC Transplants.- Clinical Aspects of Autologous Blood Stem Cell Transplantations - Review of Indications.- V. Current Developments in PBSCT.- Transplantation with Enriched Stem Cell Fractions.- Ex-Vivo Treatment of Graft by Long-Term Culture.- Induction of Autologous Graft vs Host Disease: An Immunotherapeutic Approach to Eliminate Residual Leukemia After Autologous Bone Marrow Transplantation.- Stem Cell Harvesting from Umbilical Cord Blood: A New Perspective.- Epilogue.