PERIPHERAL BLOOD MONONUCLEAR CELL GENE ARRAY PROFILES IN PATIENTS WITH OVERACTIVE BLADDER (132.8)

Abstract

Abstract Introduction: Overactive bladder (OAB) confirmation of disease often requires invasive approaches. We have previously demonstrated that peripheral blood mononuclear cells (PBMC) can provide a reporter function in solid organ retroperitoneal disease. Here we investigated the utility of using PBMC as a marker for patients with OAB. Methods: 21 patients were assessed for OAB and structural integrity. Patients with a history of recent surgery or infection were excluded. Whole blood was obtained from patients and appropriately matched controls (n=6) and PBMC were separated by density centrifugation. RNA was isolated from PBMC and converted to aRNA and subjected to microarray analysis (human U133A 2.0). Results: Microarray analysis revealed that 16 genes were differentially regulated (8 upregulated and 8 downregulated) in all patients with OAB compared with controls. Sex based analysis demonstrated 74 genes differentially regulated in males (25 upregulated and 49 downregulated), and 30 in females (13 upregulated and 17 downregulated). Of these, PDGF, MFAP3L, a microfibrillar-associated protein, and TPM1 (tropomyosin) were downregulated in all sets analyzed. Conclusions: Microarray analysis revealed many genes which were differentially regulated in PBMC from OAB patients, including regulatory/structural genes; PDGF, MFAP and TPM1 may be important in regulating structural integrity of bladder and supporting tissues. These data suggest that blood derived leukocytes can provide a reporter function for patients with solid organ disease and may serve as a diagnostic marker and/or monitor response to therapy.