Periodontal tissue regeneration after low-intensity pulsed ultrasound stimulation with or without intra-marrow perforation in two-wall intra-bony defects-A pilot study in dogs.

Abstract

To evaluate the effects of low-intensity pulsed ultrasound (LIPUS) with/without intra-marrow perforation (IMP) on periodontal healing in two-wall intra-bony defects in dogs. Two-wall intra-bony defects (5mm wide, 5mm deep) were created at the distal and mesial aspects of mandibular premolars in four beagle dogs (four defects per dog). The 16 defects were divided into four treatment groups: IMP, LIPUS, IMP+LIPUS (IMP/LIPUS) and control (open flap debridement). The LIPUS and IMP/LIPUS sites received daily LIPUS exposure for 3weeks starting 1week after surgery. The animals were euthanized 4weeks after surgery for histologic evaluation. There was significantly greater new bone formation at LIPUS (2.93±0.74mm) and IMP/LIPUS (3.18±0.52mm) sites than at control sites (1.65±0.46mm). New bone area at LIPUS (6.36±2.28mm2 ) and IMP/LIPUS (6.13±1.25mm2 ) sites was significantly greater than that at control sites (2.15±1.75mm2 ). New cementum length at LIPUS sites (4.09±0.75mm) was significantly greater than that at control (2.29±1.02mm) and IMP (2.41±0.41mm) sites. No significant difference was observed between LIPUS and IMP/LIPUS sites in any histomorphometric parameter. These findings suggest that LIPUS effectively promotes periodontal regeneration in two-wall intra-bony defects in dogs.