Abstract
In this study, we constructed a composite by combining the human dental follicle cell sheet and a manual drilled porous decalcified dentin matrix that was used to construct ectopic tissue-engineered periodontal ligament-like tissues in renal capsules of nude mice. Human dental follicle cells were harvested from human lower third molars and then embedded into atemperature-sensitiveculture dish. These cells were thenplaced into frozen porousdecalcified dentin matrix sheets andinduced by 50g/mlascorbic acid. This established a "sandwichstructure" in vitro implant that was placed in nude mice under the renal capsule. The mice were sacrificed at 4 and 8weeks after implantation, andthe implants were assessed after haematoxylin-eosin staining,Massonstaining andimmunohistochemical staining. The experimental group showed a fibre structurebetweenthe dentin and HA-TCP after 4weeks. After 8weeks, the collagen fibresincreased, and the direction was perpendicular to thedentin. Immunohistochemistry showed positive staining in the osteopontin and periostin. The composite can induce ectopic bone and fibre formation, and the fibre had a certain directionality. Besides, the composite can maintain the stability of the periodontal ligamentwidth.
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