Abstract
Abstract If lymphocytes are inactivated with mitomycin C, 60Co irradiation, or ultraviolet (UV) irradiation, and treated with sodium periodate (NaIO4), they are capable of inducing transformation of autologous lymphocytes in vitro. The optimal ratio of IO4--treated stimulator cells to untreated autologous lymphocytes is 1:2 or 1:4. Maximum incorporation of tritiated thymidine (3H-TdR) in autologous mixed lymphocyte cultures (A-MLC) occurs after 3 days. As with direct NaIO4 transformation of lymphocytes, the transformation in the A-MLC can be blocked by sodium borohydride (NaBH4). Overoxidation of the cells by NaIO4 also diminishes their ability to induce an autologous mixed lymphocyte reaction (A-MLR). Incubation of the stimulator cells after treatment with NaIO4 results in a progressively declining ability to induce transformation in the responder cells. We failed to find any evidence of a blastogenic factor released into the medium by the treated cells during periods ranging from 2 hr to 72 hr after NaIO4 treatment. NaIO4 treatment of autologous red blood cells does not render them capable of inducing transformation of autologous lymphocytes.
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