Abstract
Mitomycin C/sodium periodate, (IO 4 −)-treated lymphocytes and other cells stimulate blastogenic transformation of allogeneic lymphocytes. Peak [ 3H]TdR incorporation occurs at 2–3 days, as with IO 4 −-induced autologous mixed lymphocyte reactions, in contrast to the more delayed response that occurs in the usual allogeneic mixed lymphocyte reaction. IO 4 −-treated HeLa cells, three T and three B lymphoid cell lines stimulated a rapid response. Another B lymphoid cell line stimulated only a delayed response (peak at 5–6 days) whether or not the cells were exposed to IO 4 −. IO 4 −-treated platelets and other cell lines did not stimulate lymphocytes. Glass-adherent cells (GAC) were necessary for direct IO 4 − stimulation of lymphocytes and for IO 4 −-induced autologous and allogeneic mixed lymphocytes reactions (MLR), with one exception. IO 4 −-treated HeLa cells stimulated lymphocytes in the absence of GAC, suggesting that HeLa cells and GAC possess a common helper property.
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