Abstract
A study was made of ovaries perfused in vitro with a liquid fluorocarbon that was stabilized in emulsion by a nonionic detergent to which tissue culture medium was added. A pulsatile flow was used in an organ preservation system under aseptic conditions. Glucose was utilized and lactate produced at a fairly constant rate for as long as 20 hours of perfusion. 14C-progesterone and 14C-17αOH-progesterone were synthesized from 14C-acetate and estradiol was secreted. The production of these steroids by the perfused ovary appears to be responsive to gonadotropins.
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