Performance of the organic-diffusive gradients in thin-films passive sampler for measurement of target and suspect wastewater contaminants

Abstract

Although passive sampling is widely accepted as an excellent tool for environmental monitoring, their integration with suspect or non-targeted screening by high-resolution mass spectrometry has been limited. This study describes the application of the organic-diffusive gradients in thin-films (o-DGT) passive sampler as a tool for accurate measurement of both targeted and suspect polar organic contaminants (primarily pharmaceuticals) in wastewater. First, performance of o-DGT was assessed alongside the polar organic chemical integrative sampler (POCIS) and active sampling at two wastewater treatment facilities using targeted analyses. Overall, water concentrations measured by o-DGT, POCIS, and 24-hr integrative active samples were in good agreement with each other. There were exceptions, including a systematic difference between o-DGT and POCIS at certain sites that we propose was a result of site-specific conditions and a difference in sampling rates between the two techniques. The second component of this work involved suspect screening of the o-DGT extracts using high-resolution, high mass accuracy quadrupole time-of-flight mass spectrometry (QTOF). Lamotrigine, venlafaxine, and des-methylvenlafaxine were three suspect compounds identified and selected as proof-of-concept case studies to determine the feasibility and accuracy of o-DGT for estimating water concentrations based upon predicted sampling rates using a previously validated o-DGT diffusion model. Semi-quantification of the suspect compounds was conducting using an average surrogate response factor based on the suite of compounds measured by the targeted analyses. This, combined with the modelled sampling rates provided time-weighted average wastewater concentrations of the identified suspects within a factor of 2 of the true value, confirmed by isotope dilution with mass labelled internal surrogates. To the knowledge of the authors, this work is the first to demonstrate the utility of the o-DGT passive sampler as a potential environmental screening tool that can be integrated into the rapidly advancing field of non-targeted high resolution mass spectrometry.