Performance of the edible halophyte Cakile maritima under critical magnesium and potassium thresholds

Abstract

In their natural biotope, plants are challenged with low nutrient availability which is one of the most important limiting factors for plant production. Nutrient limitation causes disruption in several metabolic pathways resulting in a decrease in plant growth and establishment. Such responses were mostly studied in glycophytes while in halophytes the data remained scare. In the present investigation, using Cakile maritima as a plant model for halophytes, we attempt to elucidate the behavior of this species under critical magnesium and potassium thresholds. Our data showed that low K or Mg supply (0.01 mM) reduced leaf number and biomass production in leaves, stems and roots while both parameters were improved at 0.05 mM Mg and K concentration before reaching an optimum at 0.25 mM Mg and 1 mM K, respectively. Several indices of K and Mg stress tolerance were reduced upon deficiency in both elements and were significantly improved by moderate K and Mg supply. Photosynthetic pigment content was reduced upon both deficiencies with the highest reductions recorded in plants suffering from severe Mg and K starvation. Increasing Mg and K supply resulted in better chlorophyll synthesis as reflected by the increase in chlorophyll stability index parameter (CSI). Furthermore, both deficiencies triggered oxidative damages as malondialdehyde content (MDA) and electrolyte leakage (EL) increased while membrane stability index (MSI) decreased under both deficiencies. Such results suggested that the cell redox homeostasis was altered when Mg and K were omitted from the nutrient solution. Interestingly, moderate and high Mg and K concentrations promoted C. maritima growth and reduced the oxidative damage caused by the lack of both elements in the external medium. Overall, C. maritima exhibited a typical halophytic behaviour, requiring less macroelements to express its growth performance when cultivated under critical magnesium and potassium thresholds.