Performance of fluorescence microspheres-based immunochromatography in simultaneous monitoring of five quinoxalines

Abstract

ABSTRACTQuinoxaline antibiotics are widely employed in the animal feeding and breeding industry and their metabolites transfer to the environment via the ecological cycle. In this study, fluorescence microspheres-based immunochromatography has been established for the simultaneous detection of five classical quinoxalines in environmental water and animal foodstuffs. Under the optimal conditions, it achieved qualitative and quantitative detection of five quinoxalines within 15 min. The visual limit of detection (LOD) for five quinoxalines in environmental water and animal foodstuffs was 5–15 µg/L and 100–250 µg/kg, respectively. Besides, assisted by a strip reader, the LOD for the quantitative detection of five quinoxalines was 0.09–2.04 µg/kg. The average recoveries for spiked samples ranged from 74.54% to 126.33%, with the coefficients of variation less than 28.21%. Thirty-four samples collected from different areas were detected with the developed immunochromatography and commercial enzyme-linked immunosorbent kits; the results of the two methods showed a good correlation.