Abstract
Whispering gallery mode resonators (WGMR) are an efficient tool for the realization of optical biosensors. A high Q factor preservation is a crucial requirement for good biosensor performances. In this work we present an Eudragit®L100 coated microspherical WGMR as an efficient immunosensor. The developed resonator was morphologically characterized using fluorescence microscopy. The functionalization process was tuned to preserve the high Q factor of the resonator. The protein binding assay was optically characterized in terms of specificity in buffer solution.
Highlights
In optical bioassays, label-based assays exploit the interaction between the analyte under study and a capturing element, labelled with a fluorescent or chemiluminescent molecule
Microspheres were treated by immersion in 10 mM Eudragit®L100 in ethanol for 1 min and in exposed to air for about 15 min, until complete solvent evaporation had occurred
The Q factor of the Whispering gallery mode resonators (WGMR) was monitored throughout the functionalization process to ensure that the chemical process and subsequent attachment of proteins preserved its optical performance [9]
Summary
Label-based assays exploit the interaction between the analyte under study and a capturing element, labelled with a fluorescent or chemiluminescent molecule. On. Sensors 2012, 12 the contrary, in a label-free approach, which is based on the change of the refractive index in the medium surrounding the optical waveguide, does not suffer this inconvenience and offers the possibility to measure the interaction between the capturing element and the analyte directly and in real time, providing the possibility of investigating dynamic interactions. Detection of immunoagents and pathogens in a medical context requires the development of both highly sensitive and selective biosensors. This increasing demand for reliable detection of biomolecules has resulted in a large variety of optical label-free biosensors [1]. Afterwards, we have tested the performance of the WGMR immunosensors in a fluidic cell
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