Abstract
Performance of Aptima E6/E7 mRNA HPV assays on fine needle aspirates from cervical lymph nodes of patients with metastatic oropharyngeal squamous cell carcinoma
Highlights
Head and neck squamous cell carcinomas (HNSCC) may be associated with high-risk human papillomavirus (HR-HPV), predominantly genotype 16 and mainly present in oropharyngeal squamous cell carcinomas (OPSCC) involving the tonsils, base of tongue (BOT) and other sites [1]
HR HPV mRNA and DNA were detected in 68.4% and 71.4% of fine needle aspirates (FNA) samples, respectively, and 96.4% were HPV genotype 16. 81% of tumor tissue samples were positive for p16 staining. 82.2% of FNA samples from patients with p16 positive tumors were positive for HPV mRNA compared to 82.4% for HPV
Agreement between FNA testing for HPV and p16 stained tumors was 84.2% (k=0.59) for mRNA compared to 81.4% (k=0.49) for DNA
Summary
Head and neck squamous cell carcinomas (HNSCC) may be associated with high-risk human papillomavirus (HR-HPV), predominantly genotype 16 and mainly present in oropharyngeal squamous cell carcinomas (OPSCC) involving the tonsils, base of tongue (BOT) and other sites [1]. During HR-HPV oncogenesis, transcription of viral oncoproteins E6 and E7 interfere with p53 and retinoblastoma (pRb) tumor suppressor pathways, a consequence of which includes the overexpression of p16 antigen [2]. The p16 immunohistochemical (IHC) test has been useful as a surrogate marker for transcriptionallyactive HPV [3,4]. HPV can be detected in SCC tissue using HPV in-situ hybridization (HPV-ISH), and commercial ISH assays have been used for detecting HPV DNA (DNA ISH) or mRNA (RNA ISH) [5]. HR-HPV [6,7,8]. Laboratory-developed tests (LDT) for HPV DNA and mRNA using PCR technology have been used on fresh, frozen and formalin-fixed paraffin-embedded (FFPE) tumor tissue [3]
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