Abstract
Fused-silica capillaries of I.D. ca 11.4 μm were coated with polyacrylate layers. The layers consisted of copolymers of silicone acrylate and ethoxyethylacrylate which were polymerized in situ by UV irradation. Capillaries with phase ratios up to 0.9 were prepared. This results in very high mass loadabilities so that solute amounts suitable for UV detection can be injected without overloading the stationary phase. This is illustrated by the separation of various mixtures containing phenolic and aromatic compounds. The retention behaviour and the kinetic performance were studied with both UV and laser-induced fluorescence detection. On a 5-m column more than 10 6 plates could be generated for anthracene. Further, the possibility of on-column preconcentration and the effect of temperature on the kinetic performance were investigated on this stationary phase.
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