Performance of a lymphocyte t interferon gamma test (Quantiferon-TB gold in tube) in the diagnosis of active tuberculosis in HIV-infected children.

Bintou Sanogo,Anselme Millogo,Makoura Barro,Alain Saga Ouermi,Boubacar Nacro,Ad Bafa Ibrahim Ouattara,Ouédraogo Abdoul Salam

doi:10.1371/journal.pone.0241789

Bintou Sanogo, Anselme Millogo + Show 5 more

Open Access

https://doi.org/10.1371/journal.pone.0241789

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Abstract

ObjectiveEvaluate the performance of QuantiFERON ® -TB Gold In-Tube test (QFT-GIT), to improve the diagnosis of active tuberculosis (TB) in Human Immuno-Deficiency Virus (HIV)-infected children.MethodSensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) of QFT-GIT were assessed in 58/63 HIV-infected children who were suspected of having TB.ResultsSensitivity of QFT-GIT was 20.69%, specificity 96.55%, PPV/NPV respectively 85.71% and 54.90%.ConclusionQFT-GIT appears to be of little contribution to the diagnosis of active TB in children living with HIV in a TB-endemic country.

Highlights

Tuberculosis is the leading opportunistic infection involved in the death of people living with Human Immuno-Deficiency Virus (HIV), justifying the importance of an early diagnosis in this group [1]
QuantiFERON ® -TB Gold In-Tube test (QFT-GIT) appears to be of little contribution to the diagnosis of active TB in children living with HIV in a TB-endemic country
Interferon gamma release assays (IGRAs) are immunodiagnostics tools in which interferon-gamma (IFN-γ) released by T-cells in response to Mycobacterium tuberculosis (M. tuberculosis)-specific antigen is measured [4,6,7,8]. They are based on in vitro stimulation of T cells using antigens specific for Mycobacterium tuberculosis, which are absent in Bacillus Calmette and Guerin (BCG) and in most atypical mycobacteria

Summary

Introduction

Tuberculosis is the leading opportunistic infection involved in the death of people living with HIV, justifying the importance of an early diagnosis in this group [1]. Performance of Quantiferon-TB gold in tube in the diagnosis of active tuberculosis in HIV-infected children quality bacteriological samples [2,3,4,5]. Interferon gamma release assays (IGRAs) are immunodiagnostics tools in which interferon-gamma (IFN-γ) released by T-cells in response to Mycobacterium tuberculosis (M. tuberculosis)-specific antigen is measured [4,6,7,8]. They are based on in vitro stimulation of T cells using antigens specific for Mycobacterium tuberculosis, which are absent in Bacillus Calmette and Guerin (BCG) and in most atypical mycobacteria. This work aims to evaluate the contribution of QFT-GIT in the diagnosis of active TB in children infected with HIV and TB suspects in Bobo Dioulasso, a TB endemic country (49 per 100000 data from 2018) [17]

Methodology

Study design and participants

Discussion