Performance in different types of chondrocytes in mandibular condylar fractures

Abstract

Objective To investigate the different types of mandibular condylar fractures effects on chondrocytes performance. Methods 3-month-old sheep were caused by mandibular condylar longitudinal and transverse fracture, respectively, after one week, three weeks, six weeks the rats were sacrificed by cervical dislocation, condylar cartilage in both groups mandibular condylar fracture and the control group were detected by immunofluorescence proliferating cell nuclear antigen (PCNA) positive cells in order to understand the different types of mandibular condylar fracture cell The proliferation, apoptosis using TdT-mediated dUTP nick end labeling (TUNEL) stain to understand the situation of different types of mandibular condylar fracture of cartilage cells, in order to understand the different types of mandibular condylar fracture of the bone after a relationship between the processes within the condylar cartilage. Results There was a significant difference, postoperative 1 week two fracture types of proliferating cell nuclear antigen (PCNA) in contrast, transverse fracture group (group A) 42.31%, vertical type group (group B) 91.30%, control group (Group C) 95.74%. Postoperative 3 weeks, 6 weeks of condylar process of longitudinal and transverse fracture of PCNA in two groups of 51.61% in group A, 48.57%, group B 82.93%, 63.16%, group C 93.02%, 75.00%. TUNEL results for 1 week after operation in group were 66.67%, 91.30%, 77.27%, postoperative 3 weeks and 6 weeks group A was 69.77%, 69.36%, B group was 86.67%, 91.49%, group C was 77.27%, 81.25%, articular condyle longitudinal fracture of apoptotic cells higher than the transverse fracture, and cell proliferation is lower than the number of transverse fracture type; decreasing the expression of postoperative one week, three weeks, six weeks are two types of fractures of the condylar cartilage into bone each marker, cartilage fall within the osteogenic capability, longitudinal fracture more obvious markers to reduce the two groups was significant difference(P<0.05). Conclusion Different types of mandibular condylar fracture in chondrocyte proliferation, there are some differences on apoptosis and endochondral bone ability to evaluate different fracture types Rehabilitation System has a guiding role. Key words: Mandibular condylar fracture; Different types of fractures; Chondrocytes; Proliferation; Apoptosis; Endochondral bone