Performance Evaluation of the VIDAS® Measles IgG Assay and Its Diagnostic Value for Measuring IgG Antibody Avidity in Measles Virus Infection

Julia Dina,Christian Creveuil,Florent Viron,Amelie Hebert,Francois Freymuth,Astrid Vabret,Stephanie Gouarin

doi:10.3390/v8080234

Julia Dina, Christian Creveuil + Show 5 more

Open Access

https://doi.org/10.3390/v8080234

Copy DOI

Abstract

The objective of this study is primarily to compare the performance of the VIDAS® Measles immunoglobulin (Ig)G assay to that of two other serological assays using an immunoassay technique, Enzygnost® Anti-measles Virus/IgG (Siemens) and Measles IgG CAPTURE EIA® (Microimmune). The sensitivity and the agreement of the VIDAS® Measles IgG assay compared to the Enzygnost® Anti-measles Virus/IgG assay and the Measles IgG CAPTURE EIA® assay are 100%, 97.2% and 99.0%, 98.4%, respectively. The very low number of negative sera for IgG antibodies does not allow calculation of specificity. As a secondary objective, we have evaluated the ability of the VIDAS® Measles IgG assay to measure anti-measles virus IgG antibody avidity with the help of the VIDAS® CMV IgG Avidity reagent, using 76 sera from subjects with measles and 238 other sera. Different groups of populations were analyzed. In the primary infection measles group, the mean IgG avidity index was 0.16 (range of 0.07 to 0.93) compared to 0.79 (range of 0.25 to 1) in the serum group positive for IgG antibodies and negative for IgM. These data allow to define a weak anti-measles virus IgG antibody avidity as an avidity index (AI) < 0.3 and a strong avidity as an AI > 0.6. The VIDAS® Measles IgG assay has a performance equivalent to that of other available products. Its use, individual and quick, is well adapted to testing for anti-measles immunity in exposed subjects.

Highlights

Measles is one of the most contagious infectious diseases, caused by the measles virus (MeV), a single-stranded RNA virus of the Paramyxoviridae family
Out of the 321 sera analyzed by the VIDAS® Measles IgG and Enzygnost® Anti-measles Virus/IgG
The performance of the VIDAS® Measles IgG assay was tested in populations with different immunological status, patients with laboratory confirmed measles, patients with measles symptoms which were not confirmed by serology and blood donors with IgG antibodies

Summary

Introduction

Measles is one of the most contagious infectious diseases, caused by the measles virus (MeV), a single-stranded RNA virus of the Paramyxoviridae family. Measles is primarily considered to be a childhood disease, it can affect people of all ages. Despite the vaccination programs adopted by many developed countries and advancements towards the goal of measles elimination, outbreaks continue to occur in Europe and more recently in the USA [1,2,3,4,5,6,7,8]. Lifelong immunity is generally reported after a wild-type measles infection, while in vaccinated people a waning immunity has been reported in correlation with lower levels or more rapid decrease of measles specific antibodies [9,10,11].

Objectives

Methods

Results

Discussion

Conclusion