Abstract
Noise and blurring in light microscope images are representative factors that affect accurate identification of cellular and subcellular structures in biological research. In this study, a method for l1-norm-based blind deconvolution after noise reduction with non-subsampled contourlet transform (NSCT) was designed and applied to a light microscope image to analyze its feasibility. The designed NSCT-based algorithm first separated the low- and high-frequency components. Then, the restored microscope image and the deblurred and denoised images were compared and evaluated. In both the simulations and experiments, the average coefficient of variation (COV) value in the image using the proposed NSCT-based algorithm showed similar values compared to the denoised image; moreover, it significantly improved the results compared with that of the degraded image. In particular, we confirmed that the restored image in the experiment improved the COV by approximately 2.52 times compared with the deblurred image, and the NSCT-based proposed algorithm showed the best performance in both the peak signal-to-noise ratio and edge preservation index in the simulation. In conclusion, the proposed algorithm was successfully modeled, and the applicability of the proposed method in light microscope images was proved based on various quantitative evaluation indices.
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